Coding
ChuA

Part:BBa_K3475000:Design

Designed by: Lu WenTing   Group: iGEM20_Tongji_China   (2020-09-26)


Heme translocating membrane protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ChuA need to work with TonB. If the chassis don't has TonB gene, it's necessary to transform it into chassis to achieve the work.


Source

Escherichia coli O157:H7

References

[1]Torres AG, Payne SM. Haem iron-transport system in enterohaemorrhagic Escherichia coli O157:H7. Mol Microbiol. 1997 Feb;23(4):825-33. doi: 10.1046/j.1365-2958.1997.2641628.x. PMID: 9157252. [2]Mammalian expression of infrared fluorescent proteins engineered from a bacterial phytochrome. Shu X, Royant A, Lin MZ, Aguilera TA, Lev-Ram V, Steinbach PA, Tsien RY Science. 2009 May 8; 324(5928):804-7. [3]Nobles CL, Clark JR, Green SI, Maresso AW. A dual component heme biosensor that integrates heme transport and synthesis in bacteria. J Microbiol Methods. 2015;118:7-17. doi:10.1016/j.mimet.2015.07.011 [4]Mimee M, Nadeau P, Hayward A, et al. An ingestible bacterial-electronic system to monitor gastrointestinalhealth. Science. 2018:360(6391):915-918. doi:10.1126/science.aas9315